Resampling Preferences

Use this page to configure the way images are re-sampled. This page also selects which transformation is being used to achieve registration.

To open the Resampling Preferences page, click to open the dialog and select the Resampling tab.

Resampling Preferences
Transformation	Specifies the mathematical mapping between the marker coordinates in the reference image and the target images. Shift: Corrects a coordinate displacement in the x and y directions. Shear: Corrects coordinate displacements, rotation, non-perpendicular axes, and unequal scale in the x and y directions.
Resampling method	Specifies the method used to compute image intensity at fractional pixel coordinates. Bi-linear: Computes a linear interpolation between pixels in the x and y directions. Bi-cubic: Computes a 4 term cubic polynomial interpolation between pixels in the x and y directions.
Reference Image	Select the reference image onto which the transformation mapping is computed. This image is not resampled.
Residuals	Opens a window listing a table of residuals from the transformation equations in all images.
Conserve Flux	Check this box to conserve flux (sigma per unit time) in the transformation equations. For example, if conserving flux and the output pixel is 1/2 as wide and 1/2 as tall, then the signal is 1/4 as high.
Verbose	Check this option to give verbose listing of registration results.

Use the Shift transformation when there is no significant rotation or scale difference between the images.
Use the Shear transformation then there are scale differences and/or rotational differences between the images.
Use the Bi-cubic resampling option when images have sharp features and many point sources.
Use the Bi-linear option if the bi-cubic filter leaves ringing artifacts. The bi-linear kernel is less sensitive to sharp points but applies more smoothing to the image. For most images, you should choose the Bi-cubic filter.